Production of alkaline protease by a genetically engineered Aspergillus oryzae U1521.
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of the alkaline protease gene of Aspergillus oryzae.
The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAA...
متن کاملSecretion of Aspergillus oryzae alkaline protease in an osmophilic yeast, Zygosaccharomyces rouxii.
To produce Aspergillus oryzae alkaline protease (Alp) in an osmophilic yeast Zygosaccharomyces rouxii, we constructed an expression plasmid consisting of the Z. rouxii glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, the prepro-Alp cDNA of A. oryzae, the whole sequence of Z. rouxii plasmid pSR1, and the G418 resistant gene. The resulting plasmid, when introduced into Z. rouxii cells, ...
متن کاملProtease production by Aspergillus oryzae in solid-state fermentation using agroindustrial substrates
BACKGROUND: An inexpensive and readily available agroindustrial substrate such as rice bran can be used to produce cheap commercial enzymes by solid-state fermentation. This work investigates the production of food-grade proteases by solid-state fermentation using readily available Thai rice bran. RESULTS: A local strain of Aspergillus oryzae (Ozykat-1) was used to produce proteases. Rice bran ...
متن کاملProduction of alkaline protease by Pseudomonas aeruginosa.
A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.
متن کاملDirect production of itaconic acid from liquefied corn starch by genetically engineered Aspergillus terreus
BACKGROUND Itaconic acid is on the DOE (Department of Energy) top 12 list of biotechnologically produced building block chemicals and is produced commercially by Aspergillus terreus. However, the production cost of itaconic acid is too high to be economically competitive with the petrochemical-based products. Itaconic acid is generally produced from raw corn starch, including three steps: enzym...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1999
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.45.99